Biochemical and molecular changes of the zosteric acid-treated Escherichia coli biofilm on a mineral surface

Abstract Purpose The main goal of the present work was to assess effectiveness zosteric acid (ZA) in hindering Escherichia coli biofilm formation on a mineral surface. Methods Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) flow system used probe situ biochemical changes induced by ZA E. sessile cells growing zinc selenide ATR plate. Comparative proteome analysis conducted better understand principal molecular that occur ZA-treated biofilms. Results treatment modified kinetics development. After exposure, dramatic carbohydrates, proteins, and DNA profiles were observed over time ATR-FTIR spectra. These results translated into physiological effects such as reduction both biomass EPS contents, inhibition growth, promotion detachment. In cells, comparative revealed that, while stress responses upregulated, pathways belonging replication repair downregulated Conclusions reduced binding capability onto ZnSe crystal, firm adhesion subsequent development variation protein patterns indicated acted factor seemed discourage proliferation, consequently decreasing surface colonization.